Figure 6. Sensitivity of strains deficient in SUN proteins to toxic compounds on fermentative YEPDA medium.

A, B, Drop assays of cells of BY4742, BY-uth1Δ, BY-p_TEF-UTH1, BY-sun4Δ, BY-sim1Δ and BY-nca3Δ strains on YEPDA plates supplemented with various drugs. Representative experiments of at least three biological replicates are presented. Significant drug effects on particular strains are marked by grey boxes; the quantification of the drug effect is shown in Figure S4 B. A, Congo red (800 µg/ml) or Calcofluor white (600 µg/ml), cells were grown at 28°C for 4 days. B, boric acid (0.2%), cells were grown at 28°C for 5 days.