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. 2013 Sep 11;8(9):e73882. doi: 10.1371/journal.pone.0073882

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© 2013 Kuznetsov et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A, Protein amounts in cell lysates (CELL), extracellular extracts (EXTRA) and the purified cell walls (CELL WALL), respectively, prepared from colonies of BY-Uth1p-HA, BY-Sun4p-HA and BY-Sim1p-HA strains. The standardized amounts of lysate-proteins were loaded onto the gel and corresponding amounts of extracellular extracts and cell wall extracts. B, Proteins from 20-times concentrated cell wall extracts used in panel A.