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. 2013 Sep 11;8(9):e74240. doi: 10.1371/journal.pone.0074240

Figure 1. S. cerevisiae cells are sensitive to ceramides.

Figure 1

(A) Survival of W303-1A cells exposed to 30 µM C2-phytoceramide (∇), 30 µM C6-ceramide (*), 30 µM C2-ceramide (▼), or equivalent volume of solvent (■). CFU values of C2-treated cells significantly different from DMSO-treated cells, P<0.0001, Two-Way ANOVA. (B) Survival of W303-1A cells exposed to 10 µM (●), 20 µM (○), 30 µM (∇-dashed lines) and 40 µM (♦-dashed lines) C2-phytoceramide, or equivalent volume of solvent (■). CFU values of C2-phytoceramide-treated cells (30 µM or 40 µM) are significantly different from DMSO-treated cells for all time points, P<0.001, Two-Way ANOVA. All CFU values (A and B) represent mean ± SE of at least 3 independent experiments, with 5 replicas in each experiment. (C) Cell cycle progression of cells exposed to 30 µM C2-phytoceramide or equivalent volume of solvent. Data from a representative experiment (of 3 independent experiments) is shown.