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. 2013 Sep 11;8(9):e74240. doi: 10.1371/journal.pone.0074240

Figure 2. S. cerevisiae cells in G2/M are more sensitive to C2-phytoceramide.

Figure 2

(A) Survival of W303-1A cells synchronized in nitrogen starvation medium. Cells were transferred to SC gal medium and then exposed to 30 µM C2-phytoceramide (▼-dashed lines) or equivalent volume of solvent (■), or maintained in nitrogen starvation medium and exposed to 30 µM C2-phytoceramide (∇-dashed lines) or equivalent volume of solvent (□). CFU values of cells transferred to SC gal and treated with 30 µM C2-phytoceramide are significantly different from C2-phytoceramide treated cells kept in nitrogen starvation medium and from DMSO-treated cells transferred or not to SC Gal, for all time points, P<0.001, Two-Way ANOVA. All CFU values represent mean ± SE of at least 3 independent experiments, with 5 replicas in each experiment. (B) Cell cycle progression of the cells described in (A). Data represent mean ± SE of at least 3 independent experiments.