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. 2013 Sep 11;8(9):e73680. doi: 10.1371/journal.pone.0073680

Figure 5. Gene expression analysis of human adipocytes after incubation with BMP7, FNDC5 and irisin.

Figure 5

Isolated preadipocytes from human subcutaneous AT of different donors were differentiated in the presence of 50/ml BMP7, 200 ng/ml FNDC5 (Abnova), and 60 ng/ml irisin (Phoenix). Gene expression of 40 genes, related to adipocyte differentiation (A) and brite differentiation (B), was assessed by a microfluidic card TaqMan gene expression assay; n ≥4, *p<0.05, **p<0.01, ***p<0.001 vs control; n.s., not significant. White bars, control; black bars, BMP7; horizontally hatched bar, FNDC5; crossed bar, irisin. Data are presented as mean values ± SEM.