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. 2013 Sep 11;8(9):e73494. doi: 10.1371/journal.pone.0073494

Figure 3. Fluorescence density of FITC-labeled astrocytes.

Figure 3

An example of a transverse section of spinal cord, in which the fluorescence density was measured, is presented in (A). This section is the same as shown in Figure 6A. Fluorescence density was measured using ImageJ at 4 points (the rectangles) in the right half and 4 points in the left half of the transverse section: 2 at the near-midline on the border of the lesion and 2 on the outer surface of the spinal cord; 2 at lateral site on the border of the lesion and 2 on the outer surface of the spinal cord.

M: midline, D: dorsal side of the spinal cord.

The graphs show the fluorescence density of astrocytes in the 1-w (B), 2-w (C) and 4-w PI (D) groups of BMSC-transplanted subgroups, and in the 4-w PI group of PBS-injected (control) subgroup (E). There is no significant difference in fluorescence density between the border of the lesion and the outer surface of the spinal cord in any of the groups.