Figure 3. Validation of the ability of cdODN-SOC to bind target transcription factors, assessed by electrophoretic mobility shift assay (EMSA).

(A) The sequences of the cdODN-SOC carrying cis-elements for Sox2, Oct4 and c-Myc and the negative control ODN with nucleotide replacements (NC–ODN). (B) Examples of EMSA images showing the ability of cdODN-SOC to bind human recombinant Sox2, Oct4 or c-Myc protein. The shift bands indicate the positions of the DNA-protein complexes and the supershift bands indicate the specific DNA-protein binding picked up by the respective antibody. Lane labels: 1, control with no proteins; 2: in the presence of cold or unlabeled cdODN-SOC; 3: in the presence of cdODN-SOC; 4: in the presence of NC–ODN; and 5: with antibody.