Figure 6. Suppression of the clonogenic ability of CSCs in h-PCCLs by cdODN-SOC.

(A) Upper left: representative examples of soft agar assays for clonogenic ability of PANC-1 CSCs under varying conditions and lower left: the amplified view of colonies. Right panel: mean data showing the effects of cdODN-SOC on the number of colonies. SP cells were more clonogenic than NSP cells and cdODN-SOC suppressed the clonogenic ability. ***p<0.001 vs SP alone. (B) Serial sphere formation assays for clonogenic ability of CSCs, showing the suppressive effects of cdODN-SOC on spherical formation. ***p<0.001 vs SP alone. Quantitatively the same results were obtained from SW1990 and BxPc-3 CSCs (data not shown).