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. 2013 Sep 11;8(9):e73942. doi: 10.1371/journal.pone.0073942

Figure 7. Inhibition of SP cell invasion and migration by cdODN-SOC.

Figure 7

(A) Invasion assay. PANC-1 CSCs were plated onto the Matrigel-coated membrane in the top chamber of the transwell and transfected or treated with gemcitabine for 48 h. All cells were mock-treated with lipofectamine 2000. Cells invaded to the lower chambered were fixed with methanol, stained with crystal violet and counted. ***p<0.001 vs SP alone; n=6. (B) Migration assay. PANC-1 CSCs were plated in the top chamber of the transwell and transfected or treated with gemcitabine for 36 h. All cells were mock-treated with lipofectamine 2000. Cells migrated to the lower chambered were fixed with methanol, stained with crystal violet and counted. ***p<0.001 vs SP alone; n=5. Quantitatively the same results were obtained from SW1990 and BxPc-3 CSCs (data not shown).