Figure 1. Effects of immersion in sugar-free, 3% sucrose, glucose, fructose and mannitol solutions on Arabidopsis seedlings and cotyledon guard cell distributions.

(A) Representative 14 day-old seedlings. Scale bar  = 1 cm. (B) Fluorescence microscopy images of abaxial cotyledon epidermis stained with FM4-64 dye. Representative images from 10–24 independent seedlings were shown. The maximum intensity projections were constructed with a 0.5 µm step size. Scale bars  = 20 μm.