Figure 2. Targeting the SnJHER 472 bp part after bacterial administration of dsJHER₄₇₂.

A. Confirmation of dsJHER₄₇₂ synthesis in IPTG induced HT115 bacteria. Agarose gel electrophoresis of RNA extracted from IPTG induced HT115/L4440 (1) and HT115/pGEM T-easy-JHER_loop (2) bacteria followed by RNase-A treatment in high salinity buffer. B. Semiquantitative RT-PCR analysis of JHER gene in randomly selected pools of 10 insects recovered from a continuous feeding assay (1^st→5^h instar d0) with the HT115 bacteria (1, 2), 6 and 15 days post recovery (PR). C. Semiquantitative RT-PCR analysis of JHER, EcR, Hsp70 and Hsc70 in randomly selected pools of 10 insects from a continuous feeding assay (5^th→6^th instar) with the HT115 bacteria (1, 2), 7 days post feeding. As reference gene it was used the Sesamia’s b-tubulin gene.