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. 2013 Sep 11;8(9):e73834. doi: 10.1371/journal.pone.0073834

Table 2. Summary of the results after hemolymph, bacterial, baculovirus-mediated dsJHER administration.

Type of administration Targeted region Time of administration Total insects* Control Total insects* (Controltreatments) Specific SnJHER silencing Phenotype
Injection 472 bp 5thd3 100 dsL4440MCS 100 Yes Absent
1276 bp 5thd3 100 dsL4440MCS 100 Yes Absent
1725 bp 5thd3 100 dsL4440MCS 100 Yes 5% of melanized/fused epidermis
472 bp 6thd9 100 dsL4440MCS 100 Yes >90% of larval-pupal intermediates type I.
1276 bp 6thd9 100 dsL4440MCS 100 Yes >90% of larval-pupal intermediates type II.
1725 bp 6thd9 100 dsL4440MCS 100 Yes >90% of larval-pupal intermediates type III.
Bacterial feeding 472 bp 1std05thd0 100 HT115-L4440 100 Yes Absent
472 bp 1std06thd9 100 HT115-L4440 100 Yes Absent
472 bp 5thd05thd7 100 HT115-L4440 100 Yes Absent
Baculovirus infection 472 bp 5thd3 200 dsLuciferase Virus 200 Yes 14% of melanized/fused epidermis
472 bp 6thd9 200 dsLuciferase Virus 200 Yes 21% of larval-pupal intermediates type 3
472 bp Survived pupae from 6thd9 infections 78 dsLuciferase Virus 62 Yes 9% of scale-less adults

The asterisk indicates the total amount of insects used, among three independent trials, both for control and experimental groups.