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(a) The rate of change of free energy with respect to GuHCl concentration (mGuHCl or d(ΔG)/d[GuHCl]) of wild-type staphylococcal nuclease and various mutants in a GLA background obtained from fluorescence monitored GuHCl titrations at different pHs. (b) Difference in mGuHCl at each pH relative to average value of mGuHCl over neutral pH range.
