Table 1.
Guanidine hydrochloride parameters of mutants in pH 7.0 phosphate buffer.
| Mutant | ΔGH20a | Cmb | mGuHClc |
|---|---|---|---|
| GLA | 8.8 | 1.53 | 5.76 |
| V66F/GLA | 8.2 | 1.47 | 5.62 |
| V66N/GLA | 4.6 | 0.70 | 6.57 |
| V66Q/GLA | 4.5 | 0.67 | 6.68 |
| V66S/GLA | 5.7 | 0.97 | 5.92 |
| V66T/GLA | 7.1 | 1.24 | 5.75 |
| V66Y/GLA | 8.2 | 1.41 | 5.82 |
| WT | 5.3 | 0.82 | 6.44 |
a
Free energy difference between native and denatured states in the absence of denaturant. Units of kcal/mol. Error estimated to be ±0.1 kcal/mol.
b
Midpoint concentration (concentration of guanidine hydrochloride at which half of the protein is denatured) in units of molar. Error estimated to be ±0.01 M.
c
Slope value (change in free energy with respect to change in guanidine hydrochloride concentration) in units of kcal·mol−1·M−1. Error is estimated to be ±0.11 kcal·mol−1·M−1.