Table 2.
Free energy of unfolding at zero denaturant concentration (ΔGH2O) of mutants in various buffers. Units of kcal/mol.
| Buffera | pH | GLA | F | G | N | Q | S | T | Y | WT |
|---|---|---|---|---|---|---|---|---|---|---|
| A | 4.5 | 7.7 | 7.3 | 3.2 | 3.1 | 3.0 | 4.7 | 6.2 | 7.2 | 3.1 |
| A | 5.0 | 8.4 | 8.0 | 4.2 | 4.2 | 4.0 | 5.6 | 6.9 | 8.1 | 4.2 |
| A | 5.5 | 8.0 | 7.7 | 3.9 | 3.9 | 3.9 | 5.3 | 6.5 | 7.7 | 4.2 |
| A | 6.0 | 8.1 | 7.9 | 4.2 | 4.1 | ND | 5.4 | 6.6 | 7.9 | 4.7 |
| A | 6.5 | 8.2 | 8.1 | 4.2 | 4.3 | 4.3 | 5.5 | 6.8 | 8.2 | 5.0 |
| A | 7.0 | 8.4 | 8.2 | 4.3 | 4.3 | 4.3 | 5.7 | 6.8 | 8.2 | 5.5 |
| A | 7.8 | 8.4 | 8.3 | ND | 4.3 | 4.4 | 5.7 | 6.9 | 8.3 | 5.4 |
| B | 7.9 | 8.6 | 8.5 | 4.4 | 4.5 | 4.4 | 5.5 | 7.0 | 8.3 | 5.5 |
| B | 8.3 | 8.7 | 8.7 | 4.3 | 4.3 | 4.4 | 5.5 | 6.6 | 8.4 | 5.5 |
| C | 9.2 | 8.1 | 8.3 | 4.1 | 3.9 | 4.1 | 5.2 | 6.7 | 7.8 | 5.3 |
| B | 9.7 | ND | 7.7 | 4.1 | 3.9 | 3.9 | 5.2 | 6.4 | ND | ND |
| C | 9.7 | 7.8 | 7.5 | 4.0 | 3.8 | 3.9 | 5.0 | 6.3 | 7.4 | 5.2 |
| C | 10.2 | 7.3 | 7.0 | 2.9 | 3.0 | 2.9 | 4.2 | 5.4 | 6.2 | 3.9 |
a
Buffer A indicates 25mM bis-tris-propane/acetic acid, Buffer B indicates 25mM bis-tris-propane/phosphoric acid, Buffer C indicates 25mM ethanolamine/hydrochloric acid. ND indicates that a denaturation was not determined in that particular buffer for that particular mutant.