. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Biophys Chem. 2013 Jul 1;0:86–94. doi: 10.1016/j.bpc.2013.06.018

Table 3.

Midpoint concentration (C_m) of mutants in various buffers. Units of M.

Buffer^a	pH	GLA	F	G	N	Q	S	T	Y	WT
A	4.5	1.40	1.37	0.52	0.52	0.49	0.80	1.11	1.30	0.54
A	5.0	1.52	1.47	0.66	0.67	0.63	0.93	1.23	1.41	0.71
A	5.5	1.43	1.39	0.60	0.60	0.57	0.86	1.15	1.34	0.67
A	6.0	1.46	1.41	0.63	0.62	ND	0.88	1.18	1.36	0.74
A	6.5	1.51	1.46	0.66	0.67	0.65	0.94	1.24	1.40	0.81
A	7.0	1.54	1.48	0.69	0.69	0.66	0.95	1.26	1.42	0.85
A	7.8	1.55	1.49	ND	0.68	0.67	0.96	1.27	1.42	0.86
B	7.9	1.58	1.55	0.69	0.74	0.67	1.00	1.30	1.46	0.90
B	8.3	1.57	1.53	0.68	0.73	0.68	1.02	1.20	1.44	0.89
C	9.2	1.48	1.49	0.64	0.66	0.61	0.90	1.21	1.33	0.81
B	9.7	ND	1.36	0.57	0.59	0.61	0.83	1.12	ND	ND
C	9.7	1.36	1.30	0.60	0.55	0.61	0.80	1.08	1.19	0.77
C	10.2	1.18	1.12	0.44	0.40	0.40	0.64	0.90	0.96	0.52

Buffer A indicates 25mM bis-tris-propane/acetic acid, Buffer B indicates 25mM bis-tris-propane/phosphoric acid, Buffer C indicates 25mM ethanolamine/hydrochloric acid. ND indicates that a denaturation was not determined in that particular buffer for that particular mutant.