Table 4.
Slope (mGuHCl) of mutants in various buffers. Units of kcal·mol−1·M−1.
| Buffera | pH | GLA | F | G | N | Q | S | T | Y | WT |
|---|---|---|---|---|---|---|---|---|---|---|
| A | 4.5 | 5.47 | 5.36 | 6.17 | 6.02 | 6.14 | 5.88 | 5.57 | 5.50 | 5.74 |
| A | 5.0 | 5.50 | 5.42 | 6.44 | 6.27 | 6.36 | 6.08 | 5.60 | 5.71 | 5.96 |
| A | 5.5 | 5.61 | 5.55 | 6.60 | 6.60 | 6.71 | 6.15 | 5.63 | 5.79 | 6.29 |
| A | 6.0 | 5.55 | 5.63 | 6.56 | 6.61 | ND | 6.19 | 5.57 | 5.82 | 6.39 |
| A | 6.5 | 5.44 | 5.54 | 6.42 | 6.38 | 6.58 | 5.90 | 5.51 | 5.85 | 6.21 |
| A | 7.0 | 5.44 | 5.51 | 6.20 | 6.26 | 6.56 | 5.93 | 5.43 | 5.76 | 6.45 |
| A | 7.8 | 5.43 | 5.56 | ND | 6.22 | 6.52 | 5.93 | 5.41 | 5.85 | 6.29 |
| B | 7.9 | 5.43 | 5.47 | 6.34 | 6.05 | 6.49 | 5.54 | 5.35 | 5.67 | 6.18 |
| B | 8.3 | 5.54 | 5.65 | 6.36 | 5.90 | 6.55 | 5.37 | 5.47 | 5.82 | 6.20 |
| C | 9.2 | 5.47 | 5.61 | 6.49 | 5.98 | 6.71 | 5.81 | 5.52 | 5.86 | 6.56 |
| B | 9.7 | ND | 5.67 | 7.15 | 6.63 | 6.37 | 6.23 | 5.67 | ND | ND |
| C | 9.7 | 5.71 | 5.76 | 6.74 | 6.91 | 6.37 | 6.26 | 5.82 | 6.20 | 6.74 |
| C | 10.2 | 6.23 | 6.24 | 6.73 | 7.60 | 7.33 | 6.56 | 6.06 | 6.49 | 7.56 |
a
Buffer A indicates 25mM bis-tris-propane/acetic acid, Buffer B indicates 25mM bis-tris-propane/phosphoric acid, Buffer C indicates 25mM ethanolamine/hydrochloric acid. ND indicates that a denaturation was not determined in that particular buffer for that particular mutant.