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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: CNS Neurol Disord Drug Targets. 2013 Sep;12(6):849–860. doi: 10.2174/18715273113129990062

Figure 2. Oxidative Stress Alone Does Not Alter IDH Expression.

Figure 2

HT-22 neural cells were exposed to 3 mM glutamate for 24 h. Whole cell extracts were harvested at 24 h and 10 μg of total protein was probed with antibodies specific for IDH1, IDH2, IDH3, α-KG, HIF1α, and HSC70. (A) IDH1, IDH2, IDH3, α-KG, and HIF1α showed no change in expression compared to control lysates (n=3). (B) Cell viability was assessed and quantified by MTT assay (n=3), showing this oxidative challenge was non-lethal (p>0.05). The data are expressed as a percent survival compared to sorbitol (“Total Kill”) and were post-hoc analyzed using a one-way ANOVA and Turkey’s multiple comparison post-hoc analysis. HT-22 cells were photo documented following treatment to visually assess cellular viability: both the control condition (C) and the 3mM glutamate condition (D) illustrate negligible changes in cellular viability while the sorbitol condition (E) exhibits appreciable cell death.