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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cytokine. 2013 Jul 15;64(1):30–34. doi: 10.1016/j.cyto.2013.06.312

Figure 1.

Figure 1

Treatment with anti-IL-10 monoclonal antibodies (MAbs) induces damage to colonic crypts and increased inflammatory cytokine production. (A) Destruction of colonic epithelial cells was observed in explant cultures in the presence of anti-IL-10 MAbs compared to media alone explants in H&E stained sections. Red arrows indicate disruption of the epithelial cell layer of the crypts. (B) Significant differences in colonic crypt breadth were observed between anti-IL-10 antibodies treated explants and controls compared to colonic crypt length in treatment groups (n=3; 11 crypts were measured for length and breadth for each sample). (C) Treatment with anti-IL-10 MAbs induces increased IFNγ, TNFα, CD107a and perforin production. The horizontal lines denote the mean frequencies (± standard errors) of each treatment group. Statistically significant differences between each treatment group are shown.