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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Drug Alcohol Depend. 2013 May 7;132(3):562–570. doi: 10.1016/j.drugalcdep.2013.04.003

Figure 5.

Figure 5

Orexin immunoreactive fibers in the VTA. (A) High magnification view showing a dense immune-reactive fiber plexus (nickel-cobalt enhanced black fibers) enmeshing a cluster of TH-positive neurons (lightly stained cell bodies in the VTA of a PN animal (left). Orexin fibers in the VTA contain numerous varicosities and frequent appositions with TH-immunoreactive cells (thick arrows) and dendrites (thin arrows). (B) VTA from control PS rat. (center). (C) Coronal hemisection schematic of cell count area (modified from Paxinos and Watson, 1998) in VTA (right). Scale bars = 50 μm (A&B). Box indicates general area where counts were made. In all cases, images were imported into Adobe Photoshop (v 5.5), where minor revisions to contrast and brightness were made.