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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Neurotoxicology. 2013 Jun 10;38:67–73. doi: 10.1016/j.neuro.2013.06.002

Fig. 1.

Fig. 1

Diet-induced iron deficiency potentiated Mn-induced neuronal apoptotic cell death in rat brain. To study Mn intoxication in vivo, pair-fed iron-deficient and control rats were intranasally instilled with 60 mg MnCl2/kg for 3 weeks. (A) Representative TUNEL staining of the olfactory bulbs in water-instilled (control), MnCl2-instilled (Mn), water-instilled iron-deficient (ID), and MnCl2-instilled iron-deficient (ID+Mn) rats. (B) Quantification of the percentage (%) of TUNEL-positive cells within the total number of cells. Empty and closed bars represent water-instilled and MnCl2-instilled rats, respectively. Data represent as mean ± SEM (n = 3–4 samples/group). *P < 0.05 vs. water-instilled group, and #P < 0.05 for control vs. iron-deficient group; two-way ANOVA.