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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Neurotoxicology. 2013 Jun 10;38:67–73. doi: 10.1016/j.neuro.2013.06.002

Fig. 6.

Fig. 6

Caspase inhibitors or a chemical chaperone prevents Mn-induced neurotoxicity enhanced by iron depletion. (A) Cells were treated with or without DFO, and simultaneously vehicle (white bar), pan-caspase inhibitor Z-VAD-FMK (gray bar, 50μM), or caspase-3 inhibitor Z-DEAD-FMK (black bar, 50μM). Cells were then treated with MnCl2 for 48 h and then cell viability was measured using MTT assay. Data represent mean ± SEM as percent of values observed in vehicle-treated cells (% of control, n = 4–5 samples/treatment). * P < 0.05 vs. vehicle; two-way ANOVA. (B) Cells were pretreated with vehicle (white bar) or 4-PBA (black bar, 1 mM) and then treated with or without 50 μM DFO. Cells were then treated with MnCl2 for 48 h and then cell viability was measured using MTT assay. Data represent mean ± SEM as percent of values observed in vehicle-treated cells (% of control, n = 4–5 samples/treatment). * P < 0.05 vs. vehicle; two-way ANOVA.