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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Discov. 2013 Jun 13;3(9):1044–1057. doi: 10.1158/2159-8290.CD-12-0592

Figure 5. SKIL induces SLUG, which is required for SKIL-induced invasion.

Figure 5

(A) A Venn diagram showing the top 50 genes correlating with either high SKIL expression or low SKIL expression and the significant intersect between these two lists (p<0.001, binomial distribution test). The overlapping genes are listed below the diagram. (B) SLUG is required for SKIL induced Matrigel invasion. Suppression of SLUG significantly reduced SKIL induced invasion (p=0.03, student’s t-test). Bars indicate average number of invaded cell. (C) Suppression of SLUG in SKIL overexpressing cells had no effect on proliferation. Graph shows cell doublings over indicated amount of time. An shRNA targeting GFP was used as negative control. (D) SKIL overexpression induced SLUG expression. (E) SKIL overexpression increased gene expression of invasion and EMT related genes of which a subset could be reversed by SLUG suppression, marked as SLUG-dependent. mRNA levels were determined by quantitative real time PCR. (F) SLUG overexpression increased gene expression of the same set of genes, which were determined to be SLUG dependent in panel E. (G) Suppression of SMAD4 significantly induced Matrigel invasion (p=0.02, student’s t-test). Suppression of SLUG reversed the invasion induced by suppression of SMAD4 (p=0.05, student’s t-test). The bars represent average number of invaded cells. The experiments in this figure were performed in HMLE-MEKDD cells except panel A. Error bars = 1 S.D.