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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Endod. 2012 Oct 24;39(1):57–61. doi: 10.1016/j.joen.2012.09.006

Figure 2.

Figure 2

CQ induces the production of IL-6, IL-8, and MMP3. (A) Supernatants from DPSCs treated or not with 250 or 500 µmol/L CQ for 3 days were collected. The secretory cytokines were detected using ELISA-based Human Antibody Array Assay 3.0. The intensity of the dots indicating the presence of corresponding cytokines was quantified using ImageJ software. The fold induction was indicated in relation to the intensity from the CQ-untreated cells. (B) The same cells were collected and subjected to conventional RT-PCR. (C) Real-time qRT-PCR was also performed in triplicate. GAPDH was used to normalize.