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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Endod. 2012 Oct 24;39(1):57–61. doi: 10.1016/j.joen.2012.09.006

Figure 3.

Figure 3

CQ inhibits the differentiation and mineralization of DPSCs. (A) DPSCs treated or not with CQ in IM were cultured for 10 days or 4 weeks, and the differentiation or mineralization potentials were evaluated using ALP (top panels) or ARS staining (bottom panels) methods, respectively. (B) The ALP activity in CQ-treated DPSCs was quantified using a microplate reader at 405 nm. (C) ARS staining was quantified using ImageJ software. (D) A similar experiment was performed using MC3T3-E1 cells, and the ARS staining was performed to examine the mineralization potential. (E) ARS staining was destained and quantified using a microplate reader at 652 nm.