Figure 2.

Spindle Orientation in Follicle Cells Does Not Depend on aPKC

(A–C) The apical polarity determinants aPKC (A, red), Crumbs-GFP (B, red), and Bazooka (C, red), disappear from the apical cortex of mitotic follicle cells, although Dlg (green) remains along the lateral cortex. Dlg-YFP is shown in (A), and anti-Dlg staining is shown in (B) and (C). pH3, shown in blue, marks mitotic cells.

(D) aPKC^psu14 mutant follicle cell clones have normal epithelial architecture. The aPKC^psu14 mutant cells are marked by the loss of GFP (green).

(E) Metaphase spindles are not misoriented in aPKC^psu14 mutant follicle cell clones (marked by the absence of GFP).

(F) Cumulative plot of metaphase spindle angles in aPKC^psu141 mutant follicle cells.

(G) An apkc^ts/apkc^k06403 egg chamber at 18° showing a cyst encapsulation defect, as well as normal tissue.

(H) Metaphase spindles are correctly oriented in an apkc^ts/apkc^k06403 egg chamber at 18°.

(I) Cumulative plot of metaphase spindle angles in apkc^ts/apkc^k06403 follicle cells at 18°.