Figure 4.

Dlg Is Required for Mitotic Spindle Orientation

(A) dlg¹⁸ mutant follicle cell clones (marked by the absence of RFP in green) maintain normal epithelial polarity until stage 6 of oogenesis, as shown by the normal localization of aPKC (red) apically and Dlg (blue) laterally.

(B) Metaphase spindles are misoriented in dlg¹⁸ mutant follicle cells.

(C) A cumulative plot of metaphase spindle angles in dlg¹⁸ mutant follicle cells. Spindle angles in dlg¹⁸ mutant cells (n = 27) differ significantly from those in the wild-type (p value of <0.005).

(D) Dlg localization is normal in mitotic pins^p62 cells. Mutant cells are marked by the absence of GFP (in green). Dlg (red) localizes to the lateral cortex in a mitotic cell, which is outlined by a white box. DAPI is in blue.

(E and F) Pins localizes to the apical and lateral cortex in dlg¹⁴ (E) and dlg¹⁸ (F) mutant cells. Mutant cells are marked by the absence of Dlg immunoreactivity (green in E) or RFP (green in F); Pins-YFP is shown in red.

(G) Pins-YFP (in red) localizes normally during mitosis in apkc^ts/apkc^k06403 at 18°.

See also Figure S3.