Table 2.

Antibodies used in immunohistochemical studies

Antibody	Isotype	Dilution	Antigen retrieval	Incubation	Source
TDP-43	Polyclonal rabbit	1:200	Microwave 10 min trisodium citrate buffer pH 6.5	60 min at room temperature	Proteintech (Manchester, UK)
FUS/TLS	Polyclonal rabbit	1:100	Microwave for 20 min 1 mM EDTA pH 8.0	48 h at 4°C	Novus (Cambridge, UK)
p62 (3/P62 Lck ligand)	Polyclonal mouse	1:200	Microwave 10 min trisodium citrate buffer pH 6.5	60 min at room temperature	BD Transduction Laboratories (Oxford, UK)
Angiogenin (MANG-1)	Mouse monoclonal	1:750	Microwave 10 min trisodium citrate buffer pH 6.5	30 min at 37°C	AbD Serotec (Kidlington, UK)
α-smooth muscle actin (1A4)	Mouse monoclonal	1:75	Dako ENVISION™ FLEX antigen retrieval solution, low pH, 20 min at 97°C	60 min at room temperature	Dako (Ely, UK)
α-actinin-2	Polyclonal rabbit	1:50	Pressure cooker in EDTA pH 8	30 min at 37°C	Lifespan Biosciences (Stoke by Clare, UK)
CD68 (PGM1)	Mouse monoclonal	1:50	Trypsin bath at 37°C 10 min, pH 7.8	30 min at room temperature	Dako (Ely, UK)