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. 2013 Jul 19;32(18):2454–2462. doi: 10.1038/emboj.2013.163

Figure 5.

Figure 5

Loss of SAMHD1 does not result in increased RNA and envelope glycoprotein expression of selected endogenous retroelements. (A) RNA from BM-DC cultures (left) or primary MEFs (right) was analysed by RT Q-PCR for the expression of retroelement transcripts. Some cells were treated overnight with 1000 U/ml IFN-A/D as indicated (left). Each bar corresponds to one DC culture or MEF cell line from an individual embryo; numbers in square brackets refer to the cell line identifier (right). Average relative expression levels compared to GAPDH mRNA from two measurements are shown; error bars represent the range. (B) Littermate animals of the indicated Samhd1 genotypes were sacrificed at the age of 9 months and RNA was extracted from lung and spleen. Retroelement transcripts were analysed as in A. Each bar corresponds to samples from an individual animal. (C) Cultured RAW264.7 cells and freshly isolated splenocytes of the indicated Samhd1 genotypes were stained with monoclonal antibody 83A25 and secondary PE-conjugated α-rat antibody and then analysed by flow cytometry. As a control, an aliquot of cells was stained with PE-α-rat alone. 83A25 reacts with the envelope glycoproteins of endogenous murine leukaemia viruses expressed in RAW264.7 cells. (A–C) Cells and mice were on the B6 background (5D6).