Figure 7.

Inp1p exhibits a polarized distribution along the cell division axis. (A, B) Peroxisome inheritance observed in budding cells expressing Inp1p-GFP, mCherry-PTS1, and either Pex3p (A) or Pex3p-V81E (B). Merged images of the red and green channels are shown. Bar, 3 μm. (C) Peroxisomes in cells expressing either Pex3p or Pex3p-V81E were tracked over the first 100 frames of Supplementary Movie 7. Peroxisomes are presented as white spheres and their trajectories as colour-coded lines (red to green, 0–40 nm/s). Bar, 3 μm. (D) Inp1p content and peroxisome speed were computed for each peroxisome and each time point for the top panel of Supplementary Movie 7. Peroxisomes are grouped into speed categories and plotted against their Inp1p content. Bars show mean±s.e.m. Figures in brackets denote the number of individual recordings per category, while the percentages of mother cell- to bud-localized peroxisomes are displayed below the bracketed numbers.