Figure 9.

A model for peroxisome population control. Multiple Inp1p molecules connect ER-bound Pex3p and peroxisomal Pex3p into an ER-peroxisome tethering complex that anchors a peroxisome to the cER of the mother cell. Recruitment of Inp1p, but not peroxisome tethering, to foci depends on the integrity of the patch containing V81 on the surface of Pex3p (A). Pulling forces exerted by Myo2p and constriction forces exerted by the peroxisome divisional machinery lead to elongation, constriction, and ultimate rupture of the peroxisome. The division process is asymmetric and may trigger the release of larger and smaller peroxisomal fragments, which contain Inp2p and are transported to the bud (B). After its release from Myo2p, the bud-localized peroxisome can attach to a tether that is newly formed by passage of Pex3p through the ER and recruitment of Inp1p by Pex3p (C).