Figure 3.

TLR signalling-induced XBP-1 mRNA splicing requires IRE1α. (A, B) Control (CTL) and IRE1α-knockout bone marrow-derived macrophages were stimulated with tunicamycin (5 g/ml) or LPS (100 ng/ml), Pam3 (100 ng/ml), or poly(I:C) (10 g/ml) for 6 h. Total RNA was isolated from these stimulated cells, and the levels of XBP-1 mRNA (unspliced: XBP-1u, spliced: XBP-1s) were analysed by semi-quantitative RT–PCR (A) and quantitative real-time RT–PCR (B). (C) RAW264.7 cells were stimulated with LPS (100 ng/ml) for 16 h before IRE1α activation was analysed using the phos-tag gel approach. Student’s t-test was used for the statistical analysis. **P<0.01, ***P<0.005; error bars represent standard deviation (SD).

Source data for this figure is available on the online supplementary information page.

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