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. 2013 Sep 11;33(37):14825–14839. doi: 10.1523/JNEUROSCI.1611-13.2013

Figure 6.

Figure 6.

Molecular abnormalities in the cortex of Df(16)A+/− mice. A, Volcano plot of the FDR-corrected log p values (y-axis) and the corresponding log2-fold change of each gene (x-axis) of the PFC gene expression profile of Df16(A)+/− mice. Blue spots represent genes within the Df16(A) deficiency. Red spots represent primary transcripts of miRNA genes. Cyan spots represent downregulated protein encoding genes. Yellow spots represent upregulated protein encoding genes. Green arrow indicates 2310044H10Rik/Mirta22 (18) (B). List of genes differentially expressed in the PFC region of Df(16)A+/− mice. C–F, Atp2a2 mRNA and protein levels in PFC and HPC of adult Df(16)A+/− mice. Atp2a2 mRNA expression levels in PFC (C) and HPC (E) of adult Df(16)A+/− mice (n = 10 for PFC, n = 7 for HPC) and their WT littermates (n = 10 for PFC, n = 5 for HPC), as assayed by qRT-PCR. p = 0.59 (PFC), p = 0.73 (HPC), Student's t test. D, F, Atp2a2 protein expression levels in synaptosomal preparations from PFC (C) and HPC (E) of adult Df(16)A+/− mice and their WT littermate mice. Top, Representative Western blot assays of Atp2a2 in PFC (C) and HPC (E) synaptosomal samples prepared from Df(16)A+/− animals and WT littermates. GAPDH is used as loading control. Bottom, Quantification of Atp2a2 protein levels in PFC (D) and HPC (F) of Df(16)A+/− and WT animals (n = 5 each genotype). p = 0.27 (PFC), p = 0.87 (HPC), Student's t test. Expression levels in mutant animals were normalized to their respective WT littermates. Results are expressed as mean ± SEM.