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SDS/PAGE/Western blot of media from keratocyte cultures using antibodies to keratocan (K), lumican (L), and decorin (D). Keratocytes were cultured in media alone (C) or in media containing either IGF-I (IG) or insulin (IN). Media was harvested on days 7, 10, and 13 and then digested with either endo-β-galactosidase, for lumican and keratocan, or chondroinase ABC, for decorin, prior to electrophoresis.
