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DNA content of keratocyte cultures. Keratocytes were plated at low density (6 well) or high density (24 well), cultured in media containing insulin starting on day 1 and harvested on days 1, 7, 10, and 13. Compared to day 1 cultures, the DNA content of cultures significantly increased for all subsequent time points whether plated at low density (p< 0.016) or high density (p< 0.003)
