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SDS/PAGE of media from keratocyte cultures. Keratocytes were cultured in media alone (C) or in media containing IGF-II (II), IGF-I (IG) or insulin (IN) and cultures were harvested on day 10. SDS/PAGE gels of the media were examined by Western blots with an antibody to procollagen type I (WB) or stained directly with blue stain (CB).
