(A) Kill curves for persister E. coli cells treated with various concentrations of AgNO3. (B) Changes in HPF fluorescence after 3 h of administering AgNO3 at various concentrations. (p<0.05 and 0.001 for 30 and 60 μM, respectively) (C) Changes in PI fluorescence after 3 h of administering different concentrations of AgNO3. (p<0.05 and 0.001 for 30 and 60 μM, respectively) (D, E) Kill curves for E. coli persister cells treated with 30 μM AgNO3, 5 μg/mL gentamicin (Gent), 3 μg/mL ofloxacin (Oflox), 10 μg/mL ampicillin (Amp), and combinations of AgNO3 with the respective antibiotics. Error bars represent mean ± SEM for at least 6 biological replicates. *** indicates a p-value of <0.001 and ** indicates a p-value of <0.05 as determined by a student t-test, indicating a significant difference from the untreated control, unless otherwise indicated.