Figure 5.

Inhibition ELISA results showing the blocking capacity of specific and nonspecific purified IgG antibodies for IgE reactivity to Dermatophagoides pteronyssinus (Dpt) allergen extract in serum pools of atopic patients. Three serum pools (I, II, and III) with different positivity for antibody classes were used as follows: pool I (IgE+, IgG1+, and IgG4−); pool II (IgE+, IgG1−, and IgG4+); and pool III (IgE+, IgG1+, and IgG4+). (a) Levels of IgE anti-Dpt expressed in absorbance (405 nm). Statistically significant differences were determined by one-way ANOVA and the Bonferroni posttest (***P < 0.0001). (b) Percentage of inhibition of IgE binding by blocking specific and nonspecific IgG antibodies in three serum pools of atopic patients. The dashed line indicates a threshold inhibition value of 35%. Statistically significant differences were determined by Student's t-test (**P < 0.01; ***P < 0.0001).