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. 2013 Aug 28;2013:782450. doi: 10.1155/2013/782450

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Copyright © 2013 D. Talukdar and T. Talukdar.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Inhibition (KCN, H₂O₂, and NaN₃) study and visualization of SOD isoforms in native PAGE of leaf extracts of Phaseolus vulgaris cv. VL 63 (mother variety) and two SOD-deficient mutant lines, sodPv 1, and sodPv 2; lane 1: mother control (no inhibitor), lane 2: mother variety (KCN), lane 3: sodPv 1 (KCN), lane 4: sodPv 2 (KCN), lane 5: mother variety (H₂O₂), lane 6: sodPv 1 (H₂O₂), lane 7: sodPv 2 (H₂O₂), lane 8: mother variety (NaN₃), lane 9: sodPv 1, and lane 10: sodPv 2. Note that complete absence of band at lane 7; Mn SOD is absent in sodPv 2 mutant, and Fe SOD and Cu/Zn SODs are inhibited by H₂O₂ treatment.