Figure 7.

Relative expressions of genes governing mRNA transcripts of four SOD isoforms in leaves of mother control (MC) variety VL 63 of Phaseolus vulgaris L., two SOD-deficient mutants, sodPv 1 and sodPv 2, and a double mutant (DM) type derived from F₂ progeny of sodPv 1 × sodPv 2 mutants under untreated, and 50 μM arsenic- (As-) treated conditions were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The mRNA levels were normalized with respect to housekeeping gene ubiquitin and are expressed relative to those of control (MC) value, which were arbitrarily given a value of 1. Data are means ± SE of five biological replicates and two technical replicates. Single asterisks denote upregulation (at least 2.0-fold), and double asterisks represent down-regulation (at least 2.0-fold) of genes with respect to control values.