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. 2013 Aug 28;2013:782450. doi: 10.1155/2013/782450

Table 1.

Oligonucleotides used for qRT-PCR analysis of target gene expressions of superoxide dismutase (SOD) isozymes in Phaseolus vulgaris L. mother cv. VL 63 and two of its SOD-deficient mutant lines. F1—forward primers and R1—reverse primers.

Target genes Oligonucleotide sequence (5′→3′) Amplicon size (bp)
Mn SOD F1-AGTCAAGTTGCAGAGTGCAATCAAGTTC- 143
R1-CAAAGTGATTGTCAATAGCCCAACCTAAAG-
Fe SOD F1-AACAAGCAAATAGCCGGAACAGAACTAAC- 127
R1-AGAAATCGTGATTCCAGACCTGAGCAG-
Cu/Zn SOD I F1-GGCTGTATGTCAACTGGACCTCATTTCA- 139
R1-TGTCAACAATGTTGATAGCAGCGGTG-
Cu/Zn SOD II F1-GGATATATGGCATCTGTAACTCATATGC- 115
R1-GCATAAGAATGCTGATAGACAGGGTC-
Ubiquitin (housekeeping gene) F1-GCTCTCCATTTGCTCCCTGTT- 144
R1-TGAGCAATTTCAGGCACCAA-