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The –NH2-modified substrate induces the activation of FAK in BMSCs. Cells were cultured on the –NH2-modified substrate (a) and the –CH3-modified substrate (b), and lysates were prepared at the indicated times. Lysates were subjected to immunoblot analysis using phosphospecific and nonactivated FAK antibodies. An aliquot of each lysate was subjected to a kinase detection assay using SDS-PAGE. GAPDH was used as a control.
