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. Author manuscript; available in PMC: 2014 Aug 8.
Published in final edited form as: Mol Cell. 2013 Jul 25;51(3):338–348. doi: 10.1016/j.molcel.2013.06.012

Figure 2. Splicing of meiotic transcripts is more efficient during meiosis than during vegetative growth.

Figure 2

(A) Expression and splicing of meiotic transcripts SPO22, MEI4, and PCH2 in wild type (+) meiotic (Meio) and vegetative cells (Veg) and in ume6Δ (Δ) vegetative cells. Marker sizes are in base pairs. PCR products representing spliced (S) and unspliced (U) are indicated. (B) Quantification of splicing from at least three biological replicates. Dark gray bar indicates splicing efficiency at t=5h after induction of meiosis; light gray bar indicates splicing efficiency in ume6Δ vegetative cells. Note that ume6Δ also derepresses MER1, which encodes a meiotic splicing fator necessary for SPO22 pre-mRNA splicing (Munding et al., 2010). Error bars represent ± 1SD.