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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Atherosclerosis. 2013 Apr 9;229(1):52–61. doi: 10.1016/j.atherosclerosis.2013.03.035

Fig. 6. SMC proliferation and collagen secretion are induced by TLR3 deficiency.

Fig. 6

(A) Cellular cholesterol content of SMCs derived from Apoe−/− and Apoe−/−Npc1−/−Tlr3−/− incubated in SMC medium. (B) SMC migration measured by wound healing assay after incubation in 10% FBS for 24 h (C) SMC proliferation measured by thymidine incorporation assay in 10% FBS or macrophage medium. (D) SMC collagen secretion measured by Sircol collagen assay. (E) mRNA expression by real-time PCR in SMCs derived from Apoe−/−, Apoe−/−Npc1−/−, and Apoe−/−Npc1−/−Tlr3−/− mice and incubated in medium for 24 h *p < 0.05 vs. Apoe−/−. fp<0.05 vs. Apoe−/−Npc1−/−. Results are mean ± SEM of triplicate experiments.