Figure 1.
(A) E.coli codon usage analysis for wildtype Drosophila melanogaster histone H4 reading frame. (B) Modified reading frame. Codons are indicated vertically. Drosophila codons with a frequency of less than 10% in E. coli are indicated in red.
(C) Western blot of recombinant histone H4k16Ac. Lane 1: uninduced expression; lane 2: induced expression of the unmodified reading frame; lane 3: induced epression of the reading frame after codon optimization. (D) Coomassie stained gel of H4K16ac expression in RNaseE mutant BL21 cells. Total extract from uninduced cells (lane 1) and from induced cells (lane 2). Lane 3: Ni-NTA bound protein.