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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: Cancer Res. 2011 Apr 20;71(12):4292–4302. doi: 10.1158/0008-5472.CAN-10-4087

Figure 3.

Figure 3

Sox2 expression correlates with FoxM1 in neuroblastoma cells. A, mRNA levels of FoxM1, Sox2, and Bmi1 detected by qRT-PCR. BE(2)-C and SK-N-BE(2)-C cells were transfected with control siRNA or FoxM1 siRNA for 72 hours. The mRNA levels were normalized to human cyclophilin mRNA, and the control groups were set to 1. B, immunoblot showing the protein level of FoxM1, Sox2, and Bmi1 from the same samples described in A. α-Tubulin was used as loading control. C, mRNA levels of FoxM1 and Sox2 detected by qRT-PCR 24 hours after transfecting BE(2)-C cells with empty vector or pCMV-FoxM1 plasmid. The mRNA levels were normalized to human cyclophilin mRNA, and the control groups were set to 1-fold. D, immunoblot showing the expression of FoxM1 and Sox2 from the samples described in C. β-Catenin and β-actin were used as loading controls.

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