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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: Cancer Res. 2011 Apr 20;71(12):4292–4302. doi: 10.1158/0008-5472.CAN-10-4087

Figure 5.

Figure 5

Decreased Sox2 expression in neural stem/progenitor cells following FoxM1 depletion. A, schematic diagram describing the experimental procedure. B, ERT2-Cre FoxM1fl/fl neural stem/progenitor cells were treated with vehicle or 50 nmol/L 4OH-tamoxifen treatment for 4 days after being dissociated into single cells. mRNA levels of FoxM1, Sox2, and Bmi1 were detected by qRT-PCR. Wild-type neural stem/progenitor cells with same treatment were used as control. The mRNA levels were normalized to mouse cyclophilin mRNA, and the vehicle control groups were set to 1-fold. C, immunoblot showing the expression of FoxM1, Bmi1, Sox2, and Nestin of ERT2-Cre FoxM1fl/fl neural stem/progenitor cells treated with vehicle or 50 nmol/L 4OH-tamoxifen. β-actin was used as loading control.

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