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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Trends Biochem Sci. 2013 Jul 16;38(9):467–475. doi: 10.1016/j.tibs.2013.06.009

Fig. 4. Nucleosomes from the HO promoter are very repressive when placed at CLN2.

Fig. 4

The native CLN2 promoter has SBF binding sites within a nucleosome depleted region, and the gene is expressed in 100% of cell cycles (top line). Mutating the SBF sites eliminates expression (line 2), while replacing the SBF sites, but within a nucleosomal context, reduces the fraction of cell cycles in which the CLN2 promoter fires (lines 3 and 4). Inserting a region of the HO promoter with SBF binding sites (indicated by orange nucleosomes) into CLN2 promoter lacking its SBF sites results in a hybrid promoter that expresses in very few cell cycles (line 4). The region of the HO promoter where the DNA with these three nucleosomes originates is indicated.