Fig. 5.

Temporal analysis of the geomentric mean EGFP intensity of clones of CHO (a) and HeLa (b) cells with the alphoid^tetO-HAC carrying different EGFP transgenes (brown no insulator; yellow with cHS4; green with gamma-satellite DNA; blue with tDNA; purple untransformed cells) during 12 weeks of culture with selection in BS⁺ medium. c EGFP expression undergoes a progressive decline over the course of 12 weeks if the gene is not flanked by insulator sequences in HeLa cells. The error bars represent the pooled standard deviation of all clones from each insulator type