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TNF-α, IL-6, and IL-1β levels increase within 4 hours of LPS administration. TNF-α, IL-6, and IL-1β levels in mouse serum, as measured by Milliplex kit, at 0 hours, 4 hours, 1 day, 2 days, and 7 days after intradermal LPS (100 µg/mouse), saline, or no injection in dorsal aspect of right hind paw. n=5 mice per group. *=p<0.05 compared to saline or no injection.
